EXPERIMENTAL STUDY ON H~+/K~+ EXCHANGE FUNCTION AND cH-K-ATPase mRNA EXPRESSION IN RENAL IMCD CELL MONOLAYER UNDER RESPIRATORY ACID-BASE DISORDERS IN RABBIT
- VernacularTitle:呼吸性酸碱失衡家兔肾脏内髓集合管细胞单层H~+/K~+交换功能及cH-K-ATP酶mRNA表达的实验研究
- Author:
Qianming XIA
;
Zhenliang XIAO
;
Jia HUANG
- Publication Type:Journal Article
- Keywords:
acidosis,respiratory;
alkalosis,respiratory;
innermedullary collecting duct cell;
H + /K + exchange;
gene expression
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the cellular pathophysiological change in kidney IMCD cell under respiratory acid base disorders. 3% or 10% CO 2 was used to initiate chronic respiratory alkalosis(ALG) or chronic respiratory acidosis(ACG) of cultured rabbit IMCD cell. 5% CO 2 was used in control group(CG). Fluorescent probe was used to measure H + /K + exchange function of these cells. In situ hybridization and RT PCR were used to measure cH K ATPase mRNA expression of these cells.The results showed that H + /K + exchange in ACG was significantly higher than that in CG ( P 0 05). It is suggested that chronic respiratory acidosis could induce an increase in H + /K + exchange function and cH K ATPase mRNA expression. However, chronic respiratory alkalosis could induce decreases in H + /K + exchange function and cH K ATPase mRNA expression with no statistical significance, the reason of which calls for further study.
