Expression of E-cadherin, β-catenin in labial salivary glands of patients with primary Sj(o)gren's syndrome
10.3760/cma.j.issn.1007-7480.2011.06.009
- VernacularTitle:原发性干燥综合征患者唇腺组织上皮型钙黏附蛋白和β-连环素的表达及超微结构改变
- Author:
Hong ZHU
;
Li WANG
;
Xuemei WANG
;
Rong ZHU
;
Caixia ZHU
;
Bo TIAN
;
Yi GONG
- Publication Type:Journal Article
- Keywords:
Sj(o)gren's syndrome;
Immunohistochemistry;
Labial salivary glands;
infrastructure
- From:
Chinese Journal of Rheumatology
2011;15(6):394-399,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of E-cadherin( E-cad), p-catenin(β-cat) in labial salivary glands of patients with primary Sj(o)gren's syndrome (pSS) in order to explore their role in pathogenesis. Methods Biopsies of labial salivary glands were obtained from 52 patients with pSS and 30 healthy controls. The immunohistochemical staining was used to detect the expression of E-cad and β-cat. Anti-SSA and anti-SSB antibodies were measured. Semi-quantitative analysis was performed by image analysis software. Ultra-structural changes was used by electron-microscopic techniques. Results ① The area of expression, optical intensity and the accumulated optical intensity of the E-cad group [(2513±1086) μm2, 0.212±0.041, 566 ±297 ] were lower than normal controls. The expression level was reduced as the increase of lymphocyte infiltration focus. ② The area of expression, the optical density and the accumulated optical density of the β- cat group [(12 324±7883) μm2, 0.113±0.031, 566±297] was lower than those of the control group. The expression level was reduced as the increase of the lymphocyte infiltration focus. ③ The E-cad expression and the p-cat expression was positively correlated in the labial gland of patients with pSS. ④ Howev-er, there was difference in the expression of E -cad and β -cat between patients with positive SSA and negative SSA antibodies. Conclusion In salivary samples, the expression of both E-cad and p-cat in patients with pSS is lower than those of the controls. Anti-SSA/SSB antibodies are important parameters of pSS and they may be involved in the pathogenesis of pSS.
