DEVELOPMENT OF AN HIGH-EFFICIENT IMMUNOMAGNETIC ISOLATOR FOR THE PURIFICATION OF CD34~+ HEMATOPOIETIC CELLS
- VernacularTitle:CD34~+造血细胞免疫磁性高效富集仪CMSI-100的研制及应用
- Author:
Yongzhi XI
;
Shuangxi ZHANG
;
Yuyin SUN
- Publication Type:Journal Article
- Keywords:
immunomagnetic separation;
neodymium iron boron;
magnetic field strength;
CD34 + hematopoietic cells
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
According to the principle of immunomagnetic separation, a novel high-efficient immunomagnetic isolator termed as CMSI 100 for the purification of human CD34 + hematopoietic cells was designed and successfully developed. To do this , neodymium iron boron having magnetic properties superior to any other permanent magnet materials was selected as the key parts of CMSI 100 isolator. The core of the magnet assembly was constructed by sandwiching mild steel bars between 4 pieces of magnet bars of neodymium iron boron. Each piece of magnet bars must be limited to the specifications of 3.5cm?2.0cm?0.3cm so that 2 700 gauss magnetic attractive force could be exerted at the magnet surface. Otherwise magnetic field above the surface of the magnet assembly is either very stronger or weaker, both of which are not beneficial to the enrichment of CD34 + hematopoietic cells. With CMSI 100 immunomagnetic isolator, more than 90% purity and over 95% viability of CD34 + hematopoietic cells could be obtained. These data indicate that CMSI 100 immunomagnetic isolator is as good as the internationally used Isolex TM 50 made by Baxter Company in USA.
