Studies of cytotoxin from guangxi cobra venom on its purification and cytotoxic effects on the nasopharyngeal and other cancer cells
- VernacularTitle:广西眼镜蛇毒细胞毒素的分离及对人鼻咽癌等细胞抑制作用的研究
- Author:
Danqing LEI
;
Qiuyan WANG
;
Mianlin LIU
;
Yuyan SHU
;
Shengxi TANG
- Publication Type:Journal Article
- Keywords:
cytotoxicin;
tumor cell line;
MTT
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the inhibitory effect of the cytotoxin (CTX) from guangxi cobra venom on human nasopharyngeal cancer cell (CNE) and other tumons cells. METHODS The cytotoxin was isolated and purified from Guangxi cobra venom by successive chromatography on Sephadex G-50 and CM Sepharose CL-6B columns. Its cytotoxic effects and does-effect relationship on human tumors cell lines were examined by MTT assay. RESULTS The inhibitory effects of CTX CM-5 on CNE, human ovarian carcinoma cell (Ho8990), uterus cervical carcinoma cell (HELA) and lymphoma (YAC) cell lines showed a definite does-effect relationship. The IC 50 (48 h in cubation) was 1.84, 2.59, 1.84 and 0.75 mg?L -1 respctively. The inhibitory effects of CTX CM-5 on CNE increased with time. The IC 50 (3 h and 24 h cubation) was 4.78 and 1.04 mg?L -1 respctively. CONCLUSION CTX from Guangxi cobra venom exhibites strong suppressive effect on cultured tumor cells line in vitro.
