Culturing rat Kupffer cell with double collagen culture
- VernacularTitle:双层夹心法培养大鼠Kupffer细胞
- Author:
Jun WANG
;
Renxiu PENG
;
Xueyan XIA
;
Jing YANG
- Publication Type:Journal Article
- Keywords:
Kupffer cell;
culture method;
double collagen culture
- From:
Chinese Pharmacological Bulletin
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To explore a long-term Kupffer cell culture method, which can best maintain the function and activity of cell and decrease the cellular damage. METHODS Lactic dehydrogenase (LDH) in the supernatants were measured to observe the severity of cellular damage ,the function of Kupffer cell was reflected with the nitrogen monoxide(NO) content and the intracellular acid phosphatases(ACP) activity. In the conditions of common culture(CC),single collagen culture(SCC) and double collagen culture(DCC), with or without serum, study the maintenance of cellular function and the severity of cellular damage. RESULTS Compared with the other two methods, the LDH contents were significantly lower( P
