Expression of MCP-1 in human mesangial cells induced by Ox-LDL is mediated by the activition of nuclear factor-KB
- VernacularTitle:氧化低密度脂蛋白刺激人肾小球系膜细胞产生单核细胞趋化蛋白-1由核因子-?B活化调控
- Author:
Huimin WANG
;
Miaozhen ZHU
- Publication Type:Journal Article
- Keywords:
Low density lipoprotein;
Monocyte chemoattractant protein-1;
Transcription factor;
Mesangial cell
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of nuclear factor-KB( NF-KB) in the expression of monocyte chemoattractant protein-i (MCP-1) in human mesangial cells induced by oxidized low density lipoprotein(Ox-LDL) . Methods Human mesangial cells were used as target cells. Levels of inhibitory KBK (IKB?) and MCP-1 protein were measured by ELISA. Activity of transcriptional factors NF-KB was determined by electrophoresis mobility shift assay ( EMSA) . Immunohistochemistry was used to detect the translocation of p65. Results Ox-LDL increased the DNA-binding activition of NF-KB and decreased the expression of IKB?. These effects were most obvious when the mesangial cells were cultured with Ox-LDL in 50 ?g/ml for 1 hour( P
