Study on changes of phospholipids on myocardial membrane during cardiopulmonary bypass
- VernacularTitle:体外循环中猫心肌细胞膜磷脂变化的实验研究
- Author:
Fangbao DING
- Publication Type:Journal Article
- Keywords:
extracorporeal circulation;
myocardial reperfusion injury;
phospholipids;
thin layer chromatography
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the ischem ia/reperfusion- induced dynamic changes of phospholipids on m yocardial mem brane during cardiopulm onary bypass(CPB) ,and to evaluate the protective effects of4 kinds of cardioplegia.Methods: One hundred and forty felines were randomized into6 groups:Group A,control;Group B,receiving no cardioplegia during ACC;Group C,D,E,F,receiving antegrade interm ittent cold crystalloid,intermittent cold blood,continuous cold blood and continuous warm blood cardioplegia,respectively.The quantities of total phospholipids,phosphatidylcholine and phosphatidylethanolamine were calculated on basis of thin layer chromatography.Results:For group C,D,E,F,a decrease in the quantities of total phospholipids,phosphatidylcholine and phosphatidylethanolamine was seen during early reperfusion, and a maximum decrease at30 - 6 0 m in reperfusion.After further reperfusion,total phospholipids,phosphatidylcholine and phosphatidylethanolamine for group C,D,E,F began to increase.The decrease of total phospholipids,phosphatidylcholine and phosphatidylethanolamine for group F was the smallest and the increase of it was the largest.Conclusion:At30 - 6 0 min reperfusion,the most significant injury of myocardial m em brane occurs.Further im provements of warm blood cardioplegia are still warrant despite of its greater m yocardial protection effects than others. [
