Expression of Bcl-2 and Caspase-3 protein in osteosarcoma and its clinical significance
10.3760/cma.j.issn.1673-4130.2009.06.008
- VernacularTitle:Bcl-2和Caspase-3在骨肉瘤中的表达及其临床意义
- Author:
Bing LIU
- Publication Type:Journal Article
- Keywords:
Genes,bcl-2;
Osteosarcoma;
Gene expression
- From:
International Journal of Laboratory Medicine
2009;30(6):542-544,547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression levels of Bcl-2 and Caspase-3 protein in osteosarcoma,and discuss the role of Bcl-2 and Caspase-3 gene in the occurrence and development of human osteosarcoma,as well the relationship between their expression levels and Ennecking staging.Methods SP immunohistochemical staining assay was applied to detecting the expression levels of Bcl-2 and Caspase-3 protein in 30 aptients with osteosarcoma,and the results were analyzed with SPSS11.0 software.Results The positive rate of Bcl-2 and Caspase-3 protein in osteosarcoma was respectively 73.3% and 86.7%.There were prominent differences of positive rate of Bcl-2 and Caspase-3 between osteosarcoma and osteochondroma groups.The expression rates of Bcl-2 and Caspase-3 protein had no significant difference among osteosarcoma Ennecking grade Ⅰ to grade Ⅲ respectively.In osteosarcoma group,no correlation existed between the expression rates of Bcl-2 and Caspase-3(P＞0.05).Conclusion Although the occurrence and development of human osteosarcoma are correlated with the dropout of apoptosis,they are implicated in a complicated course participated by multiple genes and procedures.Bcl-2 and Caspase-3 gene play a certain role partly.SP immunohistochemical staining assay shows a higher sensitivity in detecting the expression of Bcl-2 and Caspase-3 protein in osteosarcoma;however,their expression levels were not correlative to Ennecking staging.Therefore,the expression of Bcl-2 and Caspase-3 may not be used as the criteria of the malignant degree and prognosis of osteosarcoma.
