STUDY ON DIFFERENTIATION AND CHARACTERIZATION OF CULTURED HUMAN SKELETAL MUSCLE CELLS
- VernacularTitle:人骨骼肌细胞体外分化过程及特征
- Author:
Yan MA
;
Dingguo SHEN
;
Yuefe SUN
- Publication Type:Journal Article
- Keywords:
muscle, skeletall;
cells, cultured;
cell differentiation;
creatine kinase
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim was to establish the differentiating model " in vitro " and observe the characteristics of human skeletal muscle cells (HSMC). This study developed an " in vitro " technique of preparing cultures of HSMC obtained from 8 normal subjects. Culture of HSMC was identified with immunohistochemistry, and the characteristics in cytobiology , cytobiochemistry and cytomorphology were observed. CK activity and protein synthesis were measured.The results showed that cultured HSMC could highly differentiate, and there was positive correlations between protein synthesis, CK specific activity and the differentiating potency of HSMC. It suggested that the in vitro differentiation process of HSMC was similar to its in vivo differentiation process.
