Analysis of Factors Affecting the Efficiency of Establishment of Mouse Parthenogenetic Embryonic Stem Cells
- VernacularTitle:影响小鼠孤雌胚胎干细胞的建系效率因素初探
- Author:
Zhisheng CHEN
;
Haojia WU
;
Lin LIU
- Publication Type:Journal Article
- Keywords:
Mouse;
Parthenogenesis;
Embryonic stem cells
- From:
Acta Laboratorium Animalis Scientia Sinica
2010;18(1):52-55,彩6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the factor affecting the efficiency of establishment of parthenogenetic stem cells (PESCs)in mice. Methods PESC lines were derived from parthenogenetically activated blastocyst inner cell mass of different mice strains and cultured in different systems.Results The efficiency of establishing the pESC showed no significant difference in hybrid and inbred lines.But the efficiency was increased in culture systems added with ERK inhibitor or knockout serum replacement(KSR).Conclusion The efficiency of establishment of pESC was not directly related with the genetic background of mice but it was closely related to the culture systems.
