Testification and immunoactivity assay of anti-CD3/anti-glioma bispecific antibody and its effect on T lymphocyte cells
- VernacularTitle:抗CD3-抗胶质瘤双特异性抗体的鉴定、免疫活性测定及其对T淋巴细胞的作用
- Author:
Meiqing LOU
;
Yicheng LU
;
Liying LU
;
Qian SHEN
;
Qiang HUANG
;
Wenzhong WANG
;
Chen ZHU
- Publication Type:Journal Article
- Keywords:
antibodies,bispecific;
immunoactivity;
T lypmhocyte;
glioma
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study bidirectional binding ability and immunoactivity of anti-CD3/anti-glioma bispecific antibodies(BsAb) , and to study its effect on T lymphocytes cells. Methods: Elution from standardized gel column and immnuohistochemical method were used to testify the relative molecular mass of the compound. ELISA method was used to detect its bidirectional immunoactivity and potential T lymphocytes' cytotoxicity. Phenotype and shape of T lymphocytes were also observed. Results: The relative molecular mass of the compound was 110 000, and it combined with both T lymphocytes and glioma cells.Immunoactivity assay showed the compound had good bidirectional immunoactivity. It also enhanced T lympocytes anti-tumor activity. Immunoactivity of BsAb combining with T lymphocytes was 1∶32 000, and combining with glioma cells was 1∶8 000;In phenotypes, CD3 + cells were the major one when cultivated with BsAb/IL-2, CD8 + cells increased gradually with cultivation time. Conclusion: These compounds are bispecific antibodies with bidirectional combining activity to T lymphocytes and glioma cells, they also have bidirectional immunoactivity and can activate T lymphocytes
