Establishment of p53 trancated muteins group and its expression in HeLa cells
- VernacularTitle:p53截短突变群的建立及其在HeLa细胞的表达
- Author:
Yonghua LOU
;
Yan PENG
;
Binghua JIAO
;
Xianta KONG
- Publication Type:Journal Article
- Keywords:
genes,p53;
site-directed mutagenesis;
transfection;
gene expression
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish p53 trancated muteins group and analyse their expression and effects on p53 related downstream genes in HeLa cells. Methods: Using PCR-based site-directed mutagenesis technique, a group of P53 muteins with the N- and/or C-terminals differently trancated were set up. The plasmids with p53 mutein genes were used to transfect HeLa cells, the p53 muteins and some p53 related downstream genes' expression were analyzed by RT-PCR. Results: The p53 muteins were expressed in HeLa cells and they had effects on some of p53 related downstream genes. Conclusion: The P53 muteins may be a good tool to analyze P53 function and has effects on p53 related downstream genes' expression model.
