Changes of cyclin kinase inhibitor P27 in glomerulus of Thy1 glomerulonephritis in rats
- VernacularTitle:系膜增生性肾炎大鼠肾小球周期素激酶抑制剂P27的变化及意义
- Author:
Xiaobin MEI
;
Ruolan CUI
;
Congrong GAO
;
Weijie YUAN
;
Zhibi YE
- Publication Type:Journal Article
- Keywords:
cell cycle regulatory protein;
cyclin kinase inhibitor;
glomerulonephritis,mesangial proliferative
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the role of cyclin kinase inhibitor P27 on the proliferation of mesangial cell(MC) in Thy1 glomerulonephritis(GN) rats. Methods: The Thy1 model of experimental mesangial proliferative glomerulonephritis was induced by intravenous injection of rabbit anti-thymocyte plasma. The P27 protein of glomerular lysate was detected with Western blotting analysis. The P27 mRNA was determined with RT-PCR. The extracellular matrix (ECM) protein (fibronectin and type Ⅳ collagen) of glomerular lysate was examined with ELISA. Results: Glomerular P27 protein of Thy1 model rat decreased compared with that of normal rat. The level of P27 was associated with the degree of MC proliferation:P27 began to decrease on day 1 in Thy1 model, then reached the lowest on day 3 when MC proliferation was maximal, then returned to 60% of the baseline level on day 5 when MC proliferation began to resolute. Glomeruar P27 mRNA remained constant in Thy1 GN(3 d) rats. Glomerular fibronectin(Fn) and type Ⅳ collagen increased in Thy1 model: They reached the highest on day 3(P
