Effects of oxymatrine on expression of HBV DNA in HepG2.2.15 cells
- VernacularTitle:氧化苦参碱对HepG2.2.15细胞中乙型肝炎病毒DNA表达量的影响
- Author:
Wensheng XU
;
Guojun WANG
;
Xiaohui MIAO
;
Xiong CAI
- Publication Type:Journal Article
- Keywords:
oxymatrine;
hepatitis B virus;
DNA,viral
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of oxymatrine (OM) on the expression of HBV DNA in HepG2.2.15 cells and to investigate the anti-HBV mechanism of OM. Methods: The level of HBV DNA in HepG2.2.15 cells incubated with different concentrations of OM was quantified by equivalent competitive PCR combining with DNA hybridization quantitative detection technique (PCR-ELISA). The in vitro anti-viral effect of OM was evaluated by calculating the inhibiting rate. Results: OM inhibited the expression of HBV in HepG2.2.15 cells. The inhibiting rate increased with the drug concentration. The stable concentration of OM in medium was important in keeping the inhibiting rate. Conclusion: OM can inhibit the synthesis of HBV directly at the level of HBV DNA replication.
