PROTEIN EXPRESSION AND ANTIGENICITY DETECTION OF HEPATITIS C VIRUS ENVELOPE PROTEIN E2
- VernacularTitle:HCV包膜蛋白E2的表达及其抗原性的检测
- Author:
Xuanling SHI
;
Feng CAO
;
Yon DU
- Publication Type:Journal Article
- Keywords:
hepatitis C like viruses;
viral envelope proteins;
gene expression
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems.The gene that encoding.HCV E2 protein was cloned in pQE30 and pEF1/HisC.After the expression of E2 protein in E.Coli M15 and COS 7 cell,the expressed proteins were used to detect their antigenicity with ELISA and WB.The results showed that protein E2 was expressed in both prokaryotic and eucaryotic cells.Special reaction could be detected using the expressed proteins and sera from HCV infected people.The studied E2 gene could express the desired proteins in both prokaryotic and eucaryotic expression systems,and glycosylation of the E2 protein happened in COS 7 cell.
