EFFECTS OF HIGH GLUCOSE CONCENTRATION ON FIBRONECTIN SYNTHESIS OF CULTURED HUMAN GLOMERULAR MESANGIAL CELLS
- VernacularTitle:高糖促进肾小球系膜细胞纤维连接蛋白合成的实验研究
- Author:
Ribao WEI
;
Xiangmei CHEN
;
Weng LI
- Publication Type:Journal Article
- Keywords:
glucose;
glomerular mesangial cells;
fibronectins
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To observe the high glucose concentration on proliferation and fibronectin (FN) synthesis of glomerular mesangial cells (GMC),a culture of human GMC was established and the cell proliferation was assessed using MTT method and cell counting, and the changes in FN was analyzed with RT PCR and ELISA. It was found that high glucose concentration inhibited GMC proliferation and increased FN synthesis in dose and time dependent manner.The results suggest that hyperglycemia may have important role in the development of diabetic nephropathy.
