IMMUNOHISTOCHEMISTRY AND ULTRASTRUCTURE STUDIES ON THE EXPRESSION LEVEL OF P53 AND c-ERBB-2 ORTHOTOPIC TRANSPLANTATION HUMAN GASTRIC CARCINOMA IN NUDE MICE
- VernacularTitle:人胃癌裸鼠原位移植瘤P53、c-erbB-2癌基因蛋白免疫组化和超微结构的研究
- Author:
Fu LOU
;
Wei ZHAO
;
Zihon WANG
- Publication Type:Journal Article
- Keywords:
stomach neoplasms;
protein p53;
genes,erbB 2;
immunohistochemistry
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the study is to provide an ideal animal model for the research in the field of molecular pathogenesis and experimental treatment of gastric carcinoma. By the microsurgical technique, 47 human gastric carcinoma specimens were orthotopically transplanted into the gastric mucosal layer in nude mice. Its oncogenicity, invasion, metastasis and morphologic characteristics were observed and the expression of oncogene P53, c erbB 2 and raSP 21 were assessed. Three models of human gastric carcinoma, were adenocarcinoma, squamous cell carcinoma, squamous adenocarcinoma were screened from 47 cases of patients. The oncogene proteins were positively expressed and related to the growth pattern, depth of invasion and lymph node metastasis. Its ultrastructural characteristic was similar to the human original gastric cancer cells. These models are suitable and helpful for the research of the molecular pathogenesis, invasion, metastasis and experimental treatment of gastric carcinoma.
