Rapid detection of rpoB mutation in rifampin-resistant mycobacterium tuberculosis strains with Line Probe Assay
- VernacularTitle:应用线性探针分析快速检测耐利福平结核分支杆菌的rpoB基因突变
- Author:
Maoyin PANG
;
Xinhua WENG
;
Wenhong ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Resistance;
Mutation;
Line probe assay
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect rpoB mutations in rifampin resistant mycobacterium tuberculosis strains isolated from Shanghai, and to evaluate the implication of applicating line probe assay (LiPA). Methods A fragment (213bp) of rpoB gene of 58 Mycobacterium tuberculosis isolates was amplified and sequenced, 18 rifampin resistant strains and 10 susceptible strains were selected to detect mutation by LiPA. Results Mutations of rpoB gene in 17 strains of the 18 rifampin resistant isolates were found by LiPA, and there were no mutations in any of the 10 susceptible strains. The sensitivity of LiPA was 94.4% and the concordance with drug susceptibility of Mycobacterium tuberculosis was 96.4%. Conclusions LiPA is a useful method for the rapid detection of mutations of rpoB gene in rifampin resistant Mycobacterium tuberculosis with high sensitivity.
