Obtaining, analysis and cloning of a novel gene of Schistosoma japonicum
- VernacularTitle:日本血吸虫未知基因的获得、鉴定和编码区的克隆
- Author:
Yan LI
;
Xinbing YU
;
Zhongdao WU
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Expressed sequence tags;
Defender against Apoptotic Death1;
Clone;
Bioinformatics
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain and characterize a novel gene of Schistosoma japonicum. Methods Looking for a novel gene through expressed sequence tags(EST),then predicting its function with the help of software on line. Finally the novel gene was cloned into an eukaryotic plasmid pEGFP N3. Results A novel gene with complete ORF was obtained. It has homogeneity with Defender Against Apoptotic Death 1 of human and pig. Conclusions EST is a good method to find new genes and analyse them with the help of software on line and characterize their function.
