Nucleotide sequence analysis of HCV NS5 region in Shandong province
- VernacularTitle:山东省丙型肝炎病毒分离株NS5区核苷酸序列分析
- Author:
Qin WANG
;
Wenqing ZHANG
;
Hong YU
- Publication Type:Journal Article
- Keywords:
Hepatitis C virus;
Genotype;
NS5 region;
Sequence
- From:
Chinese Journal of Infectious Diseases
2001;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the genotypes and the variations of HCV isolates in some areas of Shandong province.Methods The kit of AKZO UBI EIA 4.0 assay for antibody to hepatitis C virus(HCV)was used to screen 64 clinical anti HCV Ab positive serum samples, and 54 samples of which gave positive results. With RT nested PCR that utilized highly efficient primers,a product of 319bp in the NS5 region was amplified from 12 of 28 samples.These 12 PCR products were directly cloned using TA cloning kit, and 10 independent clones were isolated and sequenced by dideoxynucleotide chain termination reaction. Ressults Every sequencing analysis followed by comparison with reported sequences in the GenBank revealed that 10 sequences were all classified as HCV type 1b. All nucleotides changes in NS5 region were due to substitution without insertion and deletion,and most of the substitution (74%)occurred in the third base of a codon; The motif of G D D and all cysteines were conserved. Conclusion The result has demonstrated that HCV 1b is predominant in some areas of Shandong province.
