Influence of plasticizers on extracellular matrix synthesis and secretion of human peritoneal mesothelial cells in vitro
- VernacularTitle:增塑剂对人腹膜间皮细胞细胞外基质合成和分泌的影响
- Author:
Huaying PEI
;
Mei WANG
;
Jiawei TANG
- Publication Type:Journal Article
- Keywords:
Diethylhexylphthalate;
Peritoneum;
Mesothelial cells;
Extracellular matrix;
Transforming growth factor ?1
- From:
Chinese Journal of Nephrology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between diethylhexylphthalate (DEHP) and peritoneal sclerosis and its possible mechanism. Methods Isolated human peritoneal mesothelial cells (HPMC) was cultured for five days in culture solution with varied doses of DEHP ７．７?of 10-6mol/L (A group), 5.4?１０－５mol/L (B group) and 2. 2?10-4 mol/L (C group) respectively. Culture solution without DEHP was as control. Total collagen synthesis and secretion was assayed by 3H-proline incorporation. ELISA method was used to detect the level of secreted fibronectin (FN) . The expression of FN mRNA , collagen ImRNA, collagen Ⅲ mRNA and TGF－?l mRNA was determined by RT-PCR. Results (1)HPMC expressed FN, laminin, collagen Ⅲ and TGF-?１. (2)DEHP stimulated FN and collagen expression with a higher level in C group compared with control(P
