Human albumin stimulates human renal tubular epithelial cells to produce monocyte chemoattractant protein-1
- VernacularTitle:人白蛋白刺激人肾小管上皮细胞表达单核细胞趋化蛋白-1
- Author:
Gang LIU
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Monocyte chemoattractantprotein-1;
Renal tubule;
Epithelial cell;
Minimal change;
Albumin
- From:
Chinese Journal of Nephrology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between human albumin stimulation and MCP-1 production of human renal tubular epithelial cells. Methods Thirteen patients with minimal change disease (MCD) were enrolled into the study. MCP-1 expression and M? infiltration in renal biopsy tissues were studied by immunohistochemistry (IHC) and in situ hybridization (ISH). In vitro experiment, proximal tubular epithelial HK-2 cells were stimulated by human serum albumin in different concentration, then MCP-1 production was detected by Western Blot. Results In renal tissues of MCD, focal positive MCP-1 was found in tubular walls and a few M infiltrated in every area including tubular walls , meanwhile a correlation of M? infiltration between interstitium and tubular lumen was studied. In cell culture experiment, with its concentration more than 2 g/L. human serum albumin could stimulate HK-2 cells to produce MCP-1. Conclusions MCP-1 expresses in the tubules of MCD patients with massive proteinuria. A few M?infiltrate in the renal tissue and move between the interstitium and the lumen of tubules. Human serum albumin can stimulate human tubular epithelial cells to produce MCP-1.
