Effects of lovastatin on the activation of NF-?B and the expression of tPA/PAI-1 of human tubular epithelial cells cultured in high glucose
- VernacularTitle:洛伐他汀对高糖时人肾小管上皮细胞核因子-?B和组织纤溶酶原激活物及其抑制物的作用
- Author:
Limeng CHEN
;
Xuewang LI
;
Jiayou LIN
- Publication Type:Journal Article
- Keywords:
Lovastatin;
Plasminogen activator;
Nuclear factor-?B;
Glucose
- From:
Chinese Journal of Nephrology
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of high glucose(HG) on the expression of PAI-1 /tPA and the activation of NF-KB in human proximal tubular cell(HKC) ,and explore whether these effects can be reversed by lovastatin. Methods Chromogenic substance was used to show the activity of tPA and PAI-1. The expression of PAI-1 mRNA was examined by RT-PCR. Immunoblotting and laser confocal microscopy were applied to examine the expression of p65 in nuclear. Results HG could up-regulated the activity of PAI-1 from(8. 23?0. 02) to (8.40?0. 07) IU/ml, and down-regulated the activity of tPA from (6. 22?0. 52) to (4. 9?0. 11) IU/ml (control vs HG, P
