Radioreponse of neurogliocytes in the hippocampus of rat brain.
- VernacularTitle:大鼠海马内神经胶质细胞放射反应性的研究
- Author:
Ye TIAN
;
Shiyao BAO
;
Chunfeng LIU
- Publication Type:Journal Article
- Keywords:
Neurogliocytes;
Hippocampus;
Radiation effect;
Immunohistochemistry;
Rat
- From:
Chinese Journal of Radiation Oncology
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the radiation effect on different kinds of neurogliocytes in the hippocampus of rat brain and its dose, time relationship.Methods The brain sections were immunohistochemically stained separately with glial fibrillary acidic protein (GFAP), 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and CD11b(OX-42), to label the astrocytes, oligodendrocytes and microglial cells respectively. The number of GFAP, CNPase, and OX-42 positive cells in hippocampus were recorded and the data were analyzed by the Student's t test. Results By comparison with the unirradiated hemibrain, the relative number of GFAP and OX-42 positive cells increased in the hippocampus and the CNPase positive cells decreased in the irradiat hemibrain. The degree of change was both dose and time related and it was most significant at three months after 30?Gy irradiation. Conclusions The quantitative variation of different neurogliocytes in the hippocampus suggests that in addition to the decreasing traditional oligodendrocyte lineage, other increasing phenotypes, such as the astrocytes and microglial cells, as well as cellular interactions may also be involved in the pathogenetic process of brain radiation injury in the early stage.
