RECOMBINANT HUMAN AUGMENTER OF LIVER REGENERATION MAY INHIBIT COLLAGEN Ⅰ AND Ⅲ GENE EXPRESSION IN HEPATIC STELLATE CELLS
- VernacularTitle:重组人肝再生增强因子可抑制肝星状细胞Ⅰ、Ⅲ型胶原的基因表达
- Author:
Aimin WANG
;
Chaoping HE
;
Ta HAN
- Publication Type:Journal Article
- Keywords:
recombinant human augmenter of liver regeneration;
hepatic stellate cell;
collagen Ⅰ and Ⅲ;
gene expression
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Hepatic stellate cell (HSC-T) was cultured in medium containing different concentrations of recombinant human augmenter of liver regeneration (hALR),and cells were collected at different incubation period. Total RNA of HSC was isolated and collagenⅠ and Ⅲ gene expression levels were measured with reverse transcription polymerase chain reaction. The results showed that, collagen Ⅰ gene expression levels of HSC in high(0 2ng/L),middle (0 02ng/L) and low (0 002ng/L) concentrations of three hALR groups were much lower than those of control group after exposure to hALR at 8h, 24h, 48h and 72h. Collagen Ⅰ gene expression levels of HSC in high dose group were also significantly lower than those of middle and low dose groups . Collagen Ⅲ gene expression levels of HSC in middle and low dose hALR group were much lower than those of control group at 24h,48h and 72h, Collagen Ⅲ gene expression levels in high dose group were significantly lower than those of control group at 8h, 24h, 48h and 72h, and were also much lower than those of middle and low dose groups. It suggested that hALR could inhibit collagen Ⅰ and Ⅲ gene expression in hepatic stellate cells.
