DETERMINATION OF LYMPHOCYTE SUBSETS IN THE PERIPHERAL BLOOD OF THE PATIENTS WITH ALLOGENCIC HAND TRANSPLANTATION
- VernacularTitle:异体手移植术后测定外周血CD3/HLA-DR及CD3/CD(16+56)的临床意义
- Author:
Xiaofei ZHENG
;
Guoxian PEI
;
Yuron QIU
- Publication Type:Journal Article
- Keywords:
transplantation, homologous;
hand;
lymphocytes;
antigens, CD3;
CD56;
receptors, IgG
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the changes in the activated T-lymphocyte CD3/HLA-DR and CD3/CD(16+56) populations in peripheral blood of the patients with allogeneic hand transplantation, lymphocytes from peripheral blood of the patients at different time points were immunologically labeled with dual color fluoresecent monoclonal antibodies CD3/CD(16+56) and CD3/HLA-DR, mono-color fluoresecent monoclonal antibody CD25. CD25, CD3/CD(16+56), and CD3/HLA-DR were determined with flow cytometry (FCM). The levels of activated T-lymphocyte (CD25 +,CD3 +/HLA-DR + ), silent T-lymphocyte [CD3 +/CD(16+56) -,CD3 +/HLA-DA - ] decreased significantly during the first week after transplantation and then increased gradually to the pre-operafive level. Nature killer cells [CD3 -/CD(16+56) +] increased significantly at the first day after transplantation, then decreased sharply and maintained a lower level. The results suggest that immunosuppressive agents have significantly effects on lymphocyte subsets in allogenaic hand transplanted patients, and dynamic determination of HLA-DR, CD3 /CD(16+56) could be valuable in immunomonitoring after allogeneichand transplantation.
