CONSTRUCTION AND IDENTIFICATION OF THE VECTOR FOR CHINESE Hp MUTANT STRAINS DELETING PATHOGENICITY ISLAND
- VernacularTitle:致中国幽门螺杆菌致病岛缺失突变载体的构建及鉴定
- Author:
Jiong LIU
;
Guoming XU
;
Zhaoshe LI
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori;
pathogenicity island;
mutation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
A vector for a mutant Chinese Helicobacter pylori (Hp) strains knock out pathogenicity island (PAI) was constructed, which was the basis to establish a Chinese Hp mutant deleting pathogenicity island. Genetic engineering techniques such as polymerase chain reaction, plasmid extraction, agarose gel electrophoresis, restriction analysis, ligation, preparation of competence cell and transformation were used to make two cloning fragments containing two end regions of PAI and a selectable chloramphenicol resistance marker between them, and then engineering the recombined fragments into pBluescript plasmid. The result showed that restriction analysis demonstrated that the engineering mutant vector had been recombined. It suggested that we constructed an engineering mutant vector which targeted to delete the PAI in Chinese Hp strains. It will be useful for addressing the role of PAI in the pathogenesis of Hp infection.
