IN VITRO ANTI-TUMOR EFFECTS OF fas GENE ON OESOPHAGEAL CANCER CELLS
- VernacularTitle:fas基因修饰食管癌细胞的体外抑瘤效应
- Author:
Weiping ZHAO
;
Yongquan SHI
;
Fan YIN
- Publication Type:Journal Article
- Keywords:
oesophagus neoplasms;
gene, fas;
gene therapy;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the inhibitory effects of fas gene transduction on oesophageal cancer cells in vitro, the eukaryotic expression vector fas pBK was constructed and transfected into oesophageal carcinoma cells EC109. Western blot results showed high expression of Fas protein in gene transfected cells Fas EC109. Cell growth curve and plating efficiency test revealed that Fas EC109 had longer population doubling time and lower plating efficiency compared with control cells. Results of MTT assay showed increased sensitivity of Fas EC109 cells to CDDP, VCR and 5 FU. All these data suggested that transgenic expression of fas gene could effectively inhibit the proliferation and increase the drug sensitivity of oesophageal carcinoma cells in vitro.
