A study of ciprofloxacin and imipenem cross resistance in Pseudomonas aeruginosa induced experimental mouse peritonitis
- VernacularTitle:体内诱导铜绿假单胞菌对环丙沙星及亚胺培南交叉耐药研究
- Author:
Zhe YUAN
;
Yonghong XIAO
;
Qinan WANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Ciprofloxacin;
Imipenem;
Cross resistance
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of cross resistant inducibility of ciprofloxacin and imipenem which led to cross resistance in Pseudomonas aeruginosa in vivo. Methods Cross resistant mutant strains were selected and induced with clinical isolates of P.aeruginosa PA5 susceptible to ciprofloxacin and imipenem by experiment of murine peritonitis. Mutation of DNA gyrase gene, drug uptake and membrance proteins of P.aeruginosa PA5 and its mutants resistant to ciprofloxacin and imipenem were examined. Results Both ciprofloxacin and imipenem could induce resistant strain of P.aeruginosa in experimental murine peritonitis, the cross resistance rates after ciprofloxacin and imipenem challenge were 3.8% and 0.98% respectively. The results of PCR SSCP showed that 3 of six cross resistant of P.aeruginosa strains had gyrA gene mutation. Electrophoresis of outer and inner membrane proteins did not exist any difference between cross resistant strains and their parent strain PA5. Fluorometric assay for ciprofloxacin uptake by bacterial cells indicated that the accumulation of ciprofloxacin in all cross resistant variants decreased to 1/2～1/3 compared with that of PA5. After chanllenge with CCCP, the drug uptake in cross resistance mutants increased to the same level as in PA5. Conclusions The results show that cross resistant strains of P. aeruginosa could be selected and induced in vivo. Active drug efflux is the major factor contributing to the cross resistance of P. aeruginosa to both quinolone and imipenem.
