Cloning and sequencing of the gene encoding extended-spectrum ?-lactamases SHV-12
- VernacularTitle:超广谱?-内酰胺酶SHV-12编码基因的克隆及序列分析
- Author:
Weilin ZHOU
;
Yagang CHEN
;
Yunsong YU
- Publication Type:Journal Article
- Keywords:
Extended spectrum ? lactamase;
Polymerase chain reaction;
Sequence analysis
- From:
Chinese Journal of Infectious Diseases
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyse the encoding gene sequence of extended spectrum ? lactamase produced by Klebsiella pneumoniae E95 strain in Zhejiang Province and identify its ESBLs subtype. Methods The gene of ESBLs produced by E95 strain was amplified by PCR. The purified PCR product was cloned into pGEM Teasy vector and then sequenced by Sanger's dideoxy chain termination composition method. Results The encoded gene of ESBLs was identified as SHV by PCR. Its PCR product had 812 nucleotides. It had the same gene sequence as the gene encoding SHV 12 discovered in Swiss. Conclusions The ESBLs produced by Klebsiella pneumoniae E95 strain isolated from a patient in Zhejiang Province is SHV 12.
