MUTATION OF p53 IN CARCINOGENESIS OF ESOPHAGEAL TUMOR CONSEQUENT TO REFLUX OF DIFFERENT GASTRO INTESTINAL SECRETIONS
- VernacularTitle:食管反流大鼠肿瘤诱发过程中p53基因突变的研究
- Author:
Wen WANG
;
Zhaoshen LI
;
Guoming XU
- Publication Type:Journal Article
- Keywords:
gastroesophageal reflux;
esophageal neoplasms;
genes,p53;
mutation
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the study is to evaluate the presence of mutations of p53 gene in RE and esophageal tumor induced by reflux of different gastro intestinal secretions. Gastroesophageal reflux(G), duodenoesophageal reflux(D) and duodeno gastroesophageal reflux(DG) model and control group(C) were reproduced by operations. All rats were given carcinogen (methyl n amyl nitrosamine) and their esophagi were harvested at 20, 26, 40 weeks after the operation. DNA was extracted and PCR amplification for 5, 6, 7, 8 exon of p53 followed by SSCP and AgNO 3 staining were used to detect mutation of p53 gene. It was showed that p53 gene mutations occurred in D and DG group at 20 weeks, and then more mutations were found at 26 and 40 weeks. The total rate of p53 mutation was 15 4% in groupc, 11 7% in G, 31 6% in D,and 33 3% in DG.The changes in the last two groups were significantly more compared with the former two groups.It is suggested that reflux of duodenal contents might promote mutations in p53 gene in esophageal mucosa, and reflux of gastric juice alone has lesser impact on p53 mutation.
