CONSTRUCTION,IDENTIFICATION AND EXPRESSION OF FRAMEWORK PLASMID pAd.HCV-CE1 OF ADENOVIRUS EXPRESSION VECTOR OF HCV-CE1
- VernacularTitle:HCVC区、E1区基因腺病毒表达载体骨架质粒pAd.HCV-CE1的构建、鉴定及表达
- Author:
Chunqiu HAO
;
Yongxing ZHOU
;
Zhihu FENG
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
core gene/E1 gene;
adenovirus;
expression vector
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
The HCV core gene and E1 gene derived from the plasmid pBRTM/HCV1 3011 by using polymerase chain reaction(PCR) was inserted into the backward position of cytomegalovirus(CMV) immediate early promotor element of framework plasmid (pAd.CMV Link 1) of adenovirus expression vector, then the recombinant plasmid pAd.HCV CE1 was obtained. The inserted DNA of pAd.HCV CE1 was confirmed to be HCV core and E1 gene by endonuclease, PCR and sequencing. HCV core gene was expressed transiently with lipofectamine 2000 coated in human hepatoblastoma 7721 cells which was confirmed by immunofluorescence. The results indicate that the recombinant framework plasmid of adenovirus expression vector pAd.HCV CE1 can express HCV core and E1 gene. This should be useful to pack adenovirus expression vector which can express HCV core and E1 gene.
