Effect of tripterine on expression of bcl-2 family, c-myc and ICE gene in HMC-1 cells
- VernacularTitle:雷公藤红素对HMC-1细胞凋亡相关基因表达的影响
- Author:
Yixiao BAO
;
Luding ZHANG
;
Li LI
;
Lianshu HAN
;
Xiantao KONG
- Publication Type:Journal Article
- Keywords:
tripterine;
mast cell;
apoptosis;
gene expression
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the molecular mechanism of HMC-1 cell apoptosis on exposure to tripterine. Methods: After the HMC-1 cells were incubated with tripterine, the expression of bcl-2, bax, bcl-X,c-myc and ICE were assayed by using immunohistochemical staining and RT-PCR. Results: The expression of bax,c-myc were up-regulated and bcl-2 down-regulated at protein level.The expression of bax,bcl-X L, especially bcl-2 were down-regulated, and ICE was up-regulated at mRNA level. Conclusion: These results suggest that apoptosis of HMC-1 cells induced by tripterine is regulated by different expression of apoptosis-related genes.
