Humoral immune response to gene vaccine against adr hepatitis B virus
- VernacularTitle:乙肝病毒adr型基因疫苗诱导小鼠体液免疫应答
- Author:
Hongbiao JING
;
Hongyu YU
;
Yimin DAI
;
Yucheng YAO
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
gene vaccine;
humoral immune response
- From:
Academic Journal of Second Military Medical University
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct HBV adr gene vaccine pCMVS 2-S and to observe the specific humoral immune responses in C57BL/6 mice after gene immunization. Methods: The HBV gene vaccine was injected into tibialis anterior muscles of C57BL/6 mice. ELISA was used to detected the anti-HBs antibody in mice sera at various time points after gene transfer. Results: Anti-HBs in sera of mice was tested positive 1 week after plasmid injected, the level of antibody peaked 4 weeks later, and kept high titer for at least 2 months. Conclusion: The good response of humoral immunity can be induced by injection of pCMVS 2-S in C57BL/6 mice.
