THE CONTENTS AND DISTRIBUTION OF MATRIX METALLPROTEINASE IN RAT ABDOMINAL AORTIC ANEURYSMS
- VernacularTitle:腹主动脉瘤组织中基质金属蛋白酶的蛋白质水平研究
- Author:
Xiang FENG
;
Zaiping JING
- Publication Type:Journal Article
- Keywords:
aortic aneurysm, abdominal;
matrix metallproteinases;
pathogenesis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the contents and distribution of matrix metallproteinases(MMP) in abdominal aortic aneurysm(AAA) on protein level, forty male Wistar rats were randomly divided into two groups, the rats of experimental group were perfused with pancreatic elastase in the isolated abdominal aorta to construct the model of AAA. Laparotomy was preformed on the 14th day after operation, the aortas were measured and harvested. The rats of control group were operated with the aortas harvested immediately. Immunohistochemical staining and computer image analysis were performed to analyze the contents and distribution of MMP 2 and MMP 9. The aortic diameter progressed to aneurysmal dimension in the experimental group. Immunohistochemistry study revealed that the MMP contents in AAA exceeded that in normal aorta obviously. The MMP 2 cotents in AAA tunica media were higher than MMP 9. It is suggested that MMP 2 plays the main role in extracellular matrix degradation of AAA tunica media.
