Expression of caspase-3 during hepatocyte apoptosis in the mice with TNF-? induced fulminant hepatic failure
- VernacularTitle:肿瘤坏死因子-?介导凋亡肝细胞内胱门蛋白酶-3的表达
- Author:
Guoqing ZANG
;
Xiaqiu ZHOU
;
Chaofu WANG
- Publication Type:Journal Article
- Keywords:
Caspase-3;
Tumor necrosis factor-?;
Hepatocyte;
Apoptosis;
Fulminant hepatic failure
- From:
Chinese Journal of Infectious Diseases
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of protein and mRNA of caspase-3 during hepatocyte apoptosis in the mice with fulminant hepatic failure (FHF). Methods Liver damage was induced by tumor necrosis factor-? (TNF-?) in D-galactosamine (GalN) sensitized BALB/c mice. Hepatocyte apoptosis was examined by electric microscopy, in site end labeling (ISEL) and DNA electrophoresis on 1.5% agarose gel. The expression of protein and mRNA of caspase-3 was detected by immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Results After 1.5 h, 3.5 h, 6 h, and 9 h of administration of GalN and TNF-?, rates of hepatocyte apoptosis were found to be 0.0%, 0.3%, 3.15% and 3.19% respectively. The rates of caspase-3 positive hepatocytes were 0.7%, 4.6% , 17.8% and 24.2% respectively. The relative expressive density of caspase-3 mRNA were 0.26%, 0.29%, 0.41% and 0.38% respectively. Conclusions The expression of caspase-3 is up regulated in the TNF-? induced FHF model and may trigger hepatocyte apoptosis and finally lead to FHF.
