INDUCTION OF APOPTOSIS OF C6 GLIOMA CELLS BY DIFFERENT DOSES OF X-RAY IRRADIATION
- VernacularTitle:X线不同剂量照射诱发C6细胞凋亡的研究
- Author:
Xiaodong MA
;
Ji ZHANG
;
Dingbia ZHOU
- Publication Type:Journal Article
- Keywords:
X ray irradiation;
C6 glioma cell line;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate whether different doses of X ray irradiation can induce apoptosis of rat C6 glioma cells. A rat C6 glioma cell was irradiated with X ray in different doses in vitro. Apoptosis was identified by the classical “ladder” pattern (oligonucleosome sized fragments) in DNA agarose gel electrophoresis. Results: The results showed that significant apoptosis was found in 15, 20 or 30 Gy groups at 4 hours and 1 day after X ray irradiation compared with control, whereas this phenomenon could only be seen vaguely in the 40 Gy group. The latter group showed more necrosis than apoptosis. It suggested that the apoptotic mode of cell death and necrosis may represent the response in this X ray irradiated rat C6 glioma cells in vitro.
