MECHANISM OF STIMULATING EFFECT OF 1,25-DIHYDROXYVITAMIN D_3 ON INDUCTION OF NEOVASCULARIZATION DURING GASTRIC CARCINOGENESIS INDUCED BY N-METHYL- N′-NITRO-N-NITROSOGUANIDINE IN RATS
- VernacularTitle:维生素D_3促进大鼠胃癌血管新生机制的研究
- Author:
Jin LI
;
Yuan YANG
- Publication Type:Journal Article
- Keywords:
calcitriol;
stomach neoplasms;
neovascularization;
endothelial growth factors
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the mechanism ofstimulating effect of 1,25 dihydroxyvitamin D 3 on induction of neovascularization during gastric carcinogenesis induced by N methyl N′ nitro N nitrosoguanidine in rats, gastric carcinogenesis in rats was induced by administration of MNNG (150mg/L) in drinking water. Four weeks after MNNG exposure, rats were fed with diet containing 1,25(OH) 2 D 3 (2.5?g/kg, 5.0?g/kg) . Animals were killed at week 16, and 32 for the study of neovascularization by cardiovascular perfusion with carbon ink and immunohistochemical staining of vascular endothelial cell growth factor (VEGF). Expression of VEGF and microvessel density in glandular stomach of rats receiving 1,25(OH) 2 D 3 ( 5.0?g/kg) in the diet dramatically increased when compared with the rats receiving MNNG alone at week 16, and the differences were more significant at week 32. Expression of VEGF was closely correlated with microvessel density. We propose that certain dose of 1,25(OH) 2 D 3 stimulated neovascularization during gastric carcinogenesis in rats induced by MNNG partly through increasing VEGF expression.
