APPLICATION OF ATLAS~(TM)HUMAN EXPRESSION ARRAY TO ASSESS DIFFERENTIAL GENE EXPRESSION IN ENDOMETRIAL CARCINOMA
- VernacularTitle:用Atlas~(TM)人表达阵列技术鉴定子宫内膜癌差异基因的表达
- Author:
Yuanguang MENG
;
Lihui WEI
;
Jianliu WANG
- Publication Type:Journal Article
- Keywords:
endometrial carcinoma;
human expression array;
gene expression;
polymerase chain reaction
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Atlas Human cDNA expression array technique was employed to study the differences in gene expression in normal and endometrial cancer tissues. In this technique, dATP? 32 P labeled cDNA from normal and tumor tissues was hybridized to identical human cDNA expression array membranes (containing 588 known genes). The results showed that the expression of endometrial cancer associated genes including IRP, Wnt 5, 5T4 oncofetal antigen precusor , and CD147, were higher compared with controls. It suggested that the differential hybridization technique of Atlas Human cDNA expression array can be a useful method to identify genes differentially expressed either in normal or endometrial cancer tissue.
