THE PROTECTIVE EFFECTS OF CYCLOSPORINE A ON CONCANAVALIN A INDUCED LIVER INJURY IN KUNMING MICE
- VernacularTitle:环孢菌素A阻断刀豆蛋白A诱发昆明小鼠肝损害的研究
- Author:
Xiuli ZHANG
;
Yizhong QIN
;
Xuli HAN
- Publication Type:Journal Article
- Keywords:
concanavalin A;
cyclosporine;
tumor necrosis factor;
hepatitis,toxic
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the possible effects of cyclosporine A(CsA) on the T cell dependent liver injury induced by Concanavalin A (Con A) in Kunming mice,25 Kunming mice were randomly divided into five groups. Con A (doses of 10mg/kg, 20mg/kg and 40mg/kg) was administered via the tail vein to duplicate the liver injury as the ConA group; CsA subcutaneously injected twice at doses of 130mg/kg 15 hours and 1 hour respectively before ConA challenge constitutes the CsA group. Phosphate buffered saline (PBS) only served as the control group. Blood samples for tumor necrosis factor alpha (TNF ?) and alanine aminotransferase (ALT) measurement were obtained at 8 hours after ConA administration. Histopathological examination was also performed for liver tissue. The results showed that ConA induced specific liver injury in Kunming mice was successfully duplicated. CsA markedly protected the mice from Con A induced liver injury by comparing the levels of serum ALT(44?16U/L vs 341?52U/L, P
