INHIBITORY EFFECTS OF HUMAN CHOLANGIOCARCINOMA CELL LINE BY RECOMBINANT ADENOVIRUSES p16 AND p53 TRANSDUCTION
- VernacularTitle:腺病毒介导的p16和p53的联合应用对胆管癌细胞QBC939的生长抑制作用
- Author:
Jianguo LU
;
Chen LIN
;
Zhiqian HUANG
- Publication Type:Journal Article
- Keywords:
cholangiocarcinoma;
genes, p16;
genes,p53;
adenovirus;
gene therapy
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the inhibitory effect of recombinant adenoviruses p16 (Ad p16) combined with Ad p53 on human cholangiocarcinoma cell line QBC939 in vitro. Expression of p16 was low and p53 was negative in QBC939 cells. High level of p16 and p53 expressions were observed in QBC939 cell line transfected with Ad p16 and Ad p53. The growth rates of the Ad p16 infected combined with Ad p53 infected QBC939 cells were inhibited, which was higher than those of the Ad p53 or close to Ad p16 transfection. Colony formation in Ad p16 with Ad p53 transfected cells greatly decreased versus Ad p16 transfected or Ad p53 transfected cells. The Ad p16, Ad p53 and Ad p16 with Ad p53 transfected cells manifested apoptosis and G 1 arrest, which were confirmed by the flow cytometry. The suppression effects mediated by expression of the exogenous p16 with p53 in tumor cell resulted mainly from apoptosis and G 1 arrest, which is higher than exogenous p16 or p53.
