THE STUDY OF TRANSACTIVATING EFFECT OF HBV X PROTEIN ON SV40 EARLY PROMOTER
- VernacularTitle:乙型肝炎病毒X蛋白反式激活SV40病毒早期启动子的研究
- Author:
Yan LIU
;
Jing DONG
;
Ju CHENG
- Publication Type:Journal Article
- Keywords:
HBV X gene;
eucaryotic expression;
transactivating effect;
SV40 early promoter
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Polymerase chain reaction was employed to amplify the HBV X gene from plasmid pCP10, and the product was cloned into pVR1012, then transfected HepG2 cells and cotransfected HepG2 cells with reporter plasmid pSV lacZ HBx protein produced by HepG2 cells was measured by ELISA method The activity of ? galactosidase was measured by a kit, which reflected the transactivating function of HBx protein The results showed that HepG2 cells transfected by pVR1012 X could express HBx protein The expression of ? galactosidase in HepG2 cells transfected by the pVR1012 X was 3 2 fold higher as that of control plasmid It is suggested that the recombinant plasmid pVR1012 X can be expressed in mammalian cell line, and has transactivating effect on SV40 early promoter
