STRUCTURES AND AFFINITY ANALYSIS OF APTAMERS TO BACILLUS ANTHRACIS SPORES
- VernacularTitle:炭疽芽孢杆菌芽孢适配子的结构与亲和性分析
- Author:
Pei ZHEN
;
Yajun SONG
;
Ji WANG
- Publication Type:Journal Article
- Keywords:
Bacillus anthracis;
aptamer genes,structral;
affinity
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the affinity of aptamers to Bacillus anthracis spore, a custom synthesized 78 mer random DNA library was subjected to 15 rounds of selection against spores of vaccine strain A.16R by using SELEX method. The selected aptamers were cloned and sequenced. Macaw 2.05 and DNAsis 2.5 package were employed to analyze the conserved sequences and second structure of the aptamers, respectively. Affinities of aptamers to the spores were visualized by biotin streptavidin horseradish peroxidase system. The results showed that affinities of the aptamers were different. The highest OD at 450nm was 1.2, and the lowest was 0.25. The second structure analysis revealed possible stem loops for binding to the spores. The conserved sequences, AGGGG, CCCCG, GGGTT and ACACT, were found and the aptamers having same conserved sequence demonstrated similar affinity to the spores.
