THE EXPRESSION AND EFFECT OF ANGIOTENSIN Ⅱ TYPE 2 RECEPTOR GENE MEDIATED BY ADENOVIRAL VECTOR ON THE BIOLOGICAL BEHAVIOUR OF RAT VASCULAR SMOOTH MUSCLE CELLS IN VITRO
- VernacularTitle:腺病毒介导血管紧张素Ⅱ2型受体基因转染对血管平滑肌细胞生物学行为的影响
- Author:
Jianping LIU
;
Guoxiang HE
;
Ta JING
- Publication Type:Journal Article
- Keywords:
angiotensin Ⅱ type 2 receptor;
vascular smooth muscle;
proliferation;
migration;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
To study the expression of angiotensin Ⅱ (AngⅡ) type 2 receptor (AT2R) and its effect on the proliferation and migration of vascular smooth muscle cell (VSMC) in rats. The recombinant adenoviral vector, AdCMV AT2R, containing rat AT2 receptor gene was constructed by homologous recombination, and then it was used to transfer AT2 receptor gene to rat VSMC in vitro. The expression of AT2R, bcl 2 and bax mRNA was detected by RT PCR and the rate of expression in VSMC was determined by flow cytometer. Cell proliferation was determined by incorporation of bromodeoxyuridine(BrdU) and 3 (4,5 dimethyl thiazol 2 yl)2,5 diphenytetrazolium bromide(MTT) respectively. The modified Boyden chamber method was used to test the migration of VSMC. Apoptosis was quantified by flow cytometer and by terminal deoxynucleotide transferase mediated dUTP nick end labeling(TUNEL) in situ. RT PCR showed that the expression of AT2R mRNA increased obviously in transferred VSMC, and the peak value of expression rate was about 89.51% at 48 hours; bax mRNA was also increased by 76.3% at the same time. When the expression of AT2R was at peak value, the OD value of MTT and BrdU incorporation were reduced by 61.4% and 51.6% respectively( P
