Fluorescent measurements of H~+/K~+ exchange in primary cultured inner medullary collecting duct cell monolayers or rabbit kidney
- VernacularTitle:原代培养家兔肾脏内髓集合管细胞单层H~+/K~+交换测定
- Author:
Qianming XIA
;
Jian HUANG
;
Zhenliang XIAO
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
kidney;
collecting duct;
cell culture;
H+ /K+ exchange;
fluorescent probe
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the fluorescent measurement technique for H+ /K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney. METHODS To measure H+ /K+ exchange in the primary cultured IMCD cell monolayers under different CO2 concentrations by the BCECF/AM fluorescent probe. RESULTS [pH], recovery rate after acid loaded in the respiratory acidosis group was 0.0620 ? 0.012, and was significantly higher than that of the control group (0.0504 ? 0.009, P 0.05). CONCLUSION The fluorescent measurements of H+ /K+ exchange in primary cultured IMCD cell monolayers of rabbit kidney has many benefits which are convenient manipulation, stable and credit result, economy and safety, this method should further be popularized.
