In vitro and vivo studies on features of rubella virus JR_ 23 strain in the infection of central nervous system
- VernacularTitle:风疹病毒JR_(23)株对中枢神经系统感染的体内和体外实验研究
- Author:
Ping YAO
;
Zhiyu WANG
;
Yongkang WANG
- Publication Type:Journal Article
- Keywords:
Rubella virus;
Central nervous system;
Pathology;
Immunology;
Human;
Mouse
- From:
Chinese Journal of Infectious Diseases
2000;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the infectious features of rubella virus (RV) JR 23 strain in central nervous system (CNS). Methods RV JR 23 strain infected human primary cerebral neural cell culture in vitro and Balb/c mice, which were given dexamethasone and cytoxan before infection, via peritoneal injection. Viral pathogenecity was observed postinfection and RV antigens were detected in human cerebral neural cells by IFA and immunohistochemical method. Cerebral tissues were observed by HE staining and ABC methods postinfection.Results JR 23 strain didn't produce cytopathic effect (CPE). The proliferation of JR 23 strain reached highest titer of 10 3TCID 50 /ml at 72 h postinfection and decreased gradually. RV antigens were positive in cerebral neural cells, especially around the nuclei. Focal cytopathic areas were observed in cerebral cortical area and so did neuron necrosis around by gliacytes formed stellitorsis, neuronophagia and glial nodule. RV antigens could be seen in all cerebral area, but most localized in cortical area. Pathological features were basically the same among the infected groups. The infection rates of de xamethasone, cytoxan group and the group without intervention were 60%、90% and 50%, repectively. Conclusion RV JR 23 strain is not cytocidal to human neural cells and the pathological lesions induced by JR 23 strain in CNS of mice are mainly focal or dotted neuron necrosis.
