FABRICATION OF BONE WITH POLY-BETA-HYDROXYBUTYRATE AND BONE MARROW STROMAL CELL EMPLOYING THE METHOD OF TISSUE ENGINEERING
- VernacularTitle:聚羟基丁酸酯与骨髓基质细胞构建组织工程骨的实验研究
- Author:
Peiliang SHI
;
Ping HU
;
Xiaoming GU
- Publication Type:Journal Article
- Keywords:
poly-beta-hydroxybutyrate;
marrow stromal cell;
tissue engineering
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the feasibility of using poly-beta-hydroxybutyrate as scaffolds for bone tissue engineering. Marrow stromal cells were harvested from New Zealand rabbit′s iliac bone. After being cultured and multiplied in vitro,with the use of dexamethasone to promote the osteoblastic phenotype of the cells, the cells were seeded into poly-beta-hydroxybutyrate. The cells/ poly-beta-hydroxybutyrate construction was implanted subcutaneously in nude mice. In control animals, poly-beta-hydroxybutyrate alone was implanted. Osteogenesis was assessed by histological and roentgenographic analysis. Four weeks after implantation, osteoid tissue had been observed in the specimens of the composite; 8 weeks after implantation, large amount of new bone had been found, and lamellar bone had been observed also. In the control, only fibrous tissue had been found. It suggested that poly-beta-hydroxybutyrate can be used as a scaffold material for bone tissue engineering.
