S-Adenosyl-L-Methionine prevents total parenteral nutrition-induced cholestasis in infected rats
- VernacularTitle:腺苷甲硫氨酸对感染大鼠全肠外营养胆汁淤积的预防
- Author:
Honghai ZHANG
;
Ning LI
;
Fangnan LIU
;
Jieshou LI
- Publication Type:Journal Article
- Keywords:
Infection;
Total parenteral nutrition;
Cholestasis;
Adenosyl methionine
- From:
Parenteral & Enteral Nutrition
1997;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives:This study was designed to observe the effect of S adenosyl L methionine (SAMet) on total parenteral nutrition (TPN) induced cholestasis in infected rats and to analyse the change of the bile acids in the bile. Methods:18 Sprague Dawley rats were divided randomly into 3 groups,control group,infection plus TPN group,and infection plus TPN and SAMet group.Each contained 6 rats.Caecum was ligated to induce intra abdominal infection.Nutritional support was maintained for 5 days.Bile was collected by cannulating catheter.The bile flow was determined.The blood samples were gathered to measure the levels of serum total bile acid(STBA),gammaglutamyltranspeptidase (GGT),alanine aminotransferase (ALT)and alkaline phosphatase (AKP).The pathological change in the liver was observed under light microscope and electron microscope.The billiary bile acid contents were determined by high performance liquid chromatographic (HPLC) method. Results:The infection plus TPN group showed that the bile flow was reduced,levels of STBA,GGT and AKP were elevated,and fat infiltration,bile capillary dilatation and bile embolism were obvious in the liver.However,the infection plus TPN and SAMet group showed that the bile flow was increased,levels of STBA,GGT,ALT and AKP were decreased,and the liver was kept normal. Conclusions:The use of SAMet is helpful to could prevent TPN induced cholestasis in infected rats.
