Preliminary study on prediction of radiosensitivity of human tumor cells with fluorescence in situ hybridization
- VernacularTitle:荧光原位杂交法预测肿瘤放射敏感性的初步研究
- Author:
Shun WANG
;
Xueguan LU
;
Chaosu HU
- Publication Type:Journal Article
- Keywords:
Fluorescence in situ hybridization;
Radiosensitivity, Chromosome aberration.
- From:
Chinese Journal of Radiation Oncology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether chromosome induced aberrations scored with fluorescence in situ hybridization (FISH) predict the radiosensitivity of human tumor cell lines. Methods Three human cell lines with different radiosensitivities(nasopharyngeal cell line CNE, lung adenocarcinoma cell line SPC, and breast adenocarcinoma cell line MCF 7) were used for clonogenic assay. Cells were irradiated with a dose of 1～8?Gy. Radiation induced aberration in a single chromosome (number 8) was measured at 24?h after irradiation of 2, 4 and 6?Gy.Results Radiation induced aberration in chromosome 8 reflected the radiosensitivity in CNE, SPC and MCF 7 cells 24 h after irradiation. It was associated with survival fraction at 2?Gy (r=0.96). Conclusion These results suggest that the radiation induced chromosome aberrations in interphase measured by FISH be a considerable promise for prediction of radiosensitivity in tumor cells.
