REGULATION OF EXPRESSION OF N-GSLS AND THE REVERSION OF MDR IN OVARIAN CANCER CELLS (COC1/DDP)
- VernacularTitle:卵巢癌细胞株COC1/DDP多药耐药逆转对中性鞘糖脂表达的调控作用
- Author:
Shengping LI
;
Lili WANG
;
Jiren ZHANG
- Publication Type:Journal Article
- Keywords:
ovarian neoplasms;
drug resistance, multiple;
neutral glycosphingolipids;
ceramides
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to evaluate the relationship between MDR of ovarian cancer and expression of neutral glycosphingolipids(N-GSLs) in ovarian cancer cell lines, the effects of TAM and VRP on the growth of COC1/DDP were assayed by MTT method. N-GSLs of the cells were isolated and purified with the modified Hakamoris method and analysed by HPTLC. The results showed that the expression of N-GSLs was different between parent cell lines and resistant cell sublines, the level of CMH was higher in COC1/DDP than in COC1. TAM and VRP could render multidrug-resistant cells sensitive to chemotherapy, while the level of CMH concomitantly was sharply decreased. It suggested that the expression of N-GSLs is associated with MDR of ovarian cancer, and CMH may be a kind of MDR related glycolipids in ovarian cancer.
